Description
TransTaq® DNA Polymerase High Fidelity (TransTaq®HiFi DNA Polymerase) contains TransTaq®-T DNA Polymerase and a proofreading 3’-5’ exonuclease.TransTaq® HiFi DNA Polymerase provides higher specificity and higher amplification efficiency than TransTaq®-T DNA Polymerase. Two different buffers are provided in the kit.TransTaq® HiFi Buffer I is optimized for the amplification of genomic DNA and TransTaq® HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.
- TransTaq® HiFi DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
- Extension rate is about 1-2 kb/min.
- Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
- Amplification of genomic DNA fragment up to 15 kb.
Applications
- Complex templates
- GC/AT rich templates
- Long PCR
- High yield PCR
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
| Component | AP131-01/11 | AP131-02/12 | AP131-03/13 |
| TransTaq® HiFi DNA Polymerase | 250 U×1 | 500 U×1 | 500 U×6 |
| 10×TransTaq® HiFi Buffer I | 1.2 ml×1 | 1.2 ml×1 | 1.2 ml×6 |
| 10×TransTaq® HiFi Buffer II | 1.2 ml×1 | 1.2 ml×1 | 1.2 ml×6 |
| 2.5 mM dNTP | -/400 μl×1 | -/800 μl×1 | -/800 μl×6 |
| 10×GC Enhancer | 200 μl×1 | 400 μl×1 | 1 ml×1 |
| 6×DNA Loading Buffer | 500 μl×1 | 1 ml×1 | 1 ml×2 |
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2 Du J, Wang X, Li Y, et al. DHA exhibits synergistic therapeutic efficacy with cisplatin to induce ferroptosis in pancreatic ductal adenocarcinoma via modulation of iron metabolism[J]. Cell Death & Disease, 2021.(IF 8.40)
3 Di Y Q, Han X L, Kang X L, et al. Autophagy triggers CTSD (cathepsin D) maturation and localization inside cells to promote apoptosis[J]. Autophagy, 2021.(IF 9.77)
4 Li S, Li X, Xue W, et al. Screening for functional circular RNAs using the CRISPR–Cas13 system[J]. Nature methods, 2021.(IF 30.82)
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