Description
TransStart® KD Plus DNA Polymerase is a genetically modified high fidelity DNA polymerase. This enzyme provides higher amplification capability than traditional Pfu DNA polymerase and fast amplification speed equal to Taq DNA polymerase (1 kb/min). Due to strong 3’-5’ exonuclease activity, this enzyme offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.
Applications
• Fast, high specificity amplification
• High fidelity, high yield amplification
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
| Component | AP301-01/11 | AP301-02/12 | AP301-03/13 |
| TransStart® KD Plus DNA Polymerase | 100 U×1 | 200 U×1 | 200 U×6 |
| 5×TransStart® KD Plus Buffer | 1.2 ml×1 | 1.2 ml ×2 | 1.2 ml ×12 |
| 2.5 mM dNTPs | – / 500 μl×1 | – / 1 ml ×1 | – / 1 ml ×6 |
| 50 mM MgSO4 | 200 μl×1 | 400 μl ×1 | 1 ml ×1 |
| 6×DNA Loading Buffer | 500 μl×1 | 1 ml ×1 | 1 ml ×2 |
1 Wang Z L, Gao H M, Wang S, et al. Dissection of the general two-step di-C-glycosylation pathway for the biosynthesis of (iso) schaftosides in higher plants[J]. Proceedings of the National Academy of Sciences, 2020.(IF 9.41)
2 Zhang M, Li F D, Li K, et al. Functional characterization and structural basis of an efficient di-C-glycosyltransferase from Glycyrrhiza glabra[J]. Journal of the American Chemical Society, 2020.(IF 14.61)
Reviews
There are no reviews yet.