Description
TransScript® II Multiplex Probe One-Step qRT-PCR SuperMix UDG is designed for one-step qRT-PCR with high sensitivity, high synthesis efficiency and high amplification efficiency. This kit firstly synthesizes first-strand cDNA with RNA as templates using reverse gene-specific primers, and then performs qPCR with the synthesized cDNA as templates using both forward and reverse gene-specific primers and fluorescent probes to achieve one step from reverse transcription to qPCR in a single tube. After special optimization, this kit enables consistently amplifying multiple targets in a single reaction ensuring multiplex one-step RT-qPCR. dUTP/ UDG is included in the kit to degrade dU-containing ssDNA and dsDNA, which can prevent cross contamination.
Features
• Use TransScript®II Multiplex Probe One-Step Enzyme Mix UDG, 2x PerfectStart Multiplex Probe One-Step Reaction Mix to efficiently synthesize RNA into first-strand cDNA for qPCR. It is easy to operate and reduces the chance of contamination during operation.
• Using UDG enzyme and dUTP to effectively prevent cross-contamination of PCR products with accurate data.
• High sensitivity , high specificity and the data are accurate.
Applications
• Singleplex to 4-plex RT-qPCR detection.
• High-copy and low-copy gene detection.
• RNA templates with high GC content or complex secondary structure.
• Detection of RNA virus or trace amounts of RNA.
Storage
-20°C for two years
Shipping
Dry ice (-70°C)
High sensitivity, high amplification efficiency
Using a gradient dilution (5×106 copies/mL – 5×102 copies/mL, 10-fold dilution) of the SARS-CoV-2 in vitro transcribed RNA standard and a constant concentration of Hela cell total RNA mixture as templates, amplify ORF1a, N, and RNase P genes (reference gene) with TransGen products. The results indicate that TransGen products have high amplification efficiency and sensitivity.
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