Description
TransNGS® Single Cell Full Length cDNA Synthesis&Amplification Kit is a reagent kit capable of obtaining amplified full-length cDNA from single cell. It is suitable for amplifying and constructing cDNA libraries from 1-500 cells or 10 pg-10 ng total RNA. The kit uses Single Cell Oligo (dT) as the reverse transcription primer and employs a reverse transcriptase with high synthesis efficiency and template-switching activity to add a special sequence to the 3′ end of the cDNA, thereby obtaining full-length cDNA products. The kit is compatible with various cell types and tissue types, suitable for cell materials with varying RNA content. Typically, a single-cell library can yield 10-60 ng of amplified cDNA products.
• Strong compatibility with different cell types, suitable for cells with low RNA content (such as immune cells).
• Strong compatibility with different tissue types, suitable for tissues that are difficult to dissociate (such as brain tissue).
• High yield in constructing single-cell libraries, excellent peak shapes, high efficiency in gene detection (FPKM > 1).
Single cell TSO should be stored at -70℃ or below for one year. The other components should be stored at -18℃ or below for one year.
Dry ice (-70℃)
| Component | KC901-01 (12 rxns) | KC901-02 (24 rxns) | KC901-03 (96 rxns) |
| Single Cell Lysis Buffer | 120 μl | 240 μl | 960 μl |
| Single Cell Oligo(dT) | 12 μl | 24 μl | 96 μl |
| dNTP Mix | 12 μl | 24 μl | 96 μl |
| Ribonuclease Inhibitors | 24 μl | 48 μl | 192 μl |
| Single Cell RT Buffer II | 24 μl | 48 μl | 192 μl |
| Single Cell TSO | 6 μl | 12 μl | 48 μl |
| Single Cell Reverse Transcriptase II | 12 μl | 24 μl | 96 μl |
| 2×Single Cell Amplification SuperMix II | 300 μl | 600 μl | 3×800 μl |
| Single Cell PCR Primer | 12 μl | 24 μl | 96 μl |
| RNase-free Water | 1 ml | 2 ml | 5 ml |
| Library Elution Buffer | 300 μl | 600 μl | 2.4 ml |
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