Description
The kit is designed for the Illumina high-throughput sequencing platform to preparestrand-specific or non-strand-specific transcriptome libraries. The applicable sample is 0.1 ng-100 ng of processed RNA (mRNA captured by mRNA magnetic beads or RNA obtained after rRNA removal) to prepare a high-quality sequencing library with high yield and complete information. The kit completes the second-strand cDNA synthesis, end repair and A-tailing in one step, without the purification steps, which greatly simplifies the operation process and shortens the library preparation time, and can complete the preparation of high-quality sequencing libraries within 3 hours .
• No actinomycin D, no need to avoid light, non-toxic operation, higher strand-specific ratio.
• High library conversion rate, high library yield, and high data quality.
• Whole transcriptome sequencing
• Gene expression analysis
• Single nucleotide variation analysis
• Variable splicing testing
• Fusion genetic testing
• Analysis of non-coding RNA and RNA precursor
at -20°C for one year
Dry ice (-70℃)
| Component | KP701-01(12 rxns) | KP701-02(96 rxns) |
| 1×RNA Fragmentation Buffer | 230 μl | 2×890 μl |
| Library First-Strand Buffer III | 75 μl | 580 μl |
| Library First-Strand Enzyme Mix II | 24 μl | 192 μl |
| Library SEA Buffer III (dUTP) | 300 μl | 4×600 μl |
| Library SEA Buffer III (dNTP) | 300 μl | 4×600 μl |
| Library SEA Enzyme Mix II | 120 μl | 960 μl |
| TransNGS® Adapter for Illumina® (16 μM) | 60 μl | 480 μl |
| Adapter Dilution Buffer | 600 μl | 5 ml |
| Adapter Ligation Buffer IV | 360 μl | 4×720 μl |
| Adapter Ligation Enzyme III | 60 μl | 480 μl |
| TransNGS® Library Amplification SuperMix (2×) | 300 μl | 4×600 μl |
| TransNGS® Universal Primer for Illumina | 60 μl | 480 μl |
| Uracil-DNA Glycosylase | 12 μl | 96 μl |
| RNase-free Water | 2×1 ml | 3×5 ml |
Reviews
There are no reviews yet.