Description
TransNGS® DNA Library Prep Kit for Illumina® is developed for the Illumina high-throughput sequencing platform, designed to efficiently and rapidly construct sequencing libraries from 1 ng to 1 μg of fragmented dsDNA. This reagent kit is suitable for various types of samples, including sonication fragmented genomic DNA, enzymatic digests, nucleic acid amplification products, chromatin immunoprecipitation DNA (ChIP DNA), and DNA from paraffin-embedded tissue sections (FFPE DNA), among others. It can be used in conjunction with exon capture or other target capture reagents (including Agilent SureSelect® and Roche NimbleGen SeqCap® EZ series) for exon sequencing or targeted sequencing.
• Wide range of applicable sample types.
• High library conversion rate.
• Whole genome sequencing.
• Targeted gene sequencing.
• Exon sequencing / other target capture sequencing.
• Metagenomics sequencing.
• Immunoprecipitation sequencing.
at -18℃ or below for one year
Dry ice (-70℃)
| Component | KP201-11-V2 (12 rxns) | KP201-03-V2 (96 rxns) |
| End-repair and A-tailing Reaction Mix | 120 μl | 960 μl |
| End-repair and A-tailing Enzyme Mix | 60 μl | 480 μl |
| TransNGS® Adapter for Illumina® (16 μM)* | 60 μl | 480 μl |
| Adapter Dilution Buffer | 600 μl | 5 ml |
| Adapter-ligation Buffer III | 360 μl | 4×720 μl |
| Adapter-ligation Enzyme II | 60 μl | 480 μl |
| TransNGS® Library Amplification SuperMix (2×) | 300 μl | 4×600 μl |
| TransNGS® Universal Primer Mix for Illumina®* | 60 μl | 480 μl |
| Library Elution Buffer | 2 ml | 4×4 ml |
| Nuclease-free Water | 1 ml | 5 ml |
Reviews
There are no reviews yet.