Description
TransNGS® ATAC-Seq Library Prep Kit for Illumina® is developed for the Illumina high-throughput sequencing platform and is suitable for constructing ATAC libraries from mammalian cells. The resulting libraries can be used for Single-Read or Paired-End sequencing. This kit utilizes a novel transposase enzyme technology for library construction targeting chromatin open regions, with experiments completed in only 3 hours. It requires minimal cell input, providing high-resolution sequencing profiles with starting cell amounts ranging from 50 to 50,000 cells.
· Time saving and simple operation.
· Minimal cell input requirement. Library construction can be completed with as few as 50 cells.
Preparing chromatin open region fragments into short fragment libraries suitable for the Illumina high-throughput sequencing platform
at -20℃ for one year
Dry ice (-70℃)
| Component | KP171-01(12 rxns) | KP171-02(96 rxns) |
| Cell Lysis Buffer | 24 μl | 192 μl |
| Lysis Enhancer | 12 μl | 96 μl |
| 4×Insertion Buffer | 90 μl | 720 μl |
| Insertion Enhancer 1 | 12 μl | 96 μl |
| Insertion Enhancer 2 | 12 μl | 96 μl |
| Tn5-50 Enzyme Mix | 48 μl | 384 μl |
| 4×Tn5 Digestion Buffer | 120 μl | 960 μl |
| TransNGS ATAC-Seq Library Amplification SuperMix I（2×） | 300 μl | 4×600 μl |
| TransNGS ATAC-Seq Library Amplification SuperMix II（2×） | 300 μl | 4×600 μl |
| Library Elution Buffer | 600 μl | 5 ml |
| Nuclease-free Water | 1 ml | 2 × 5 ml |
| Tn5 Storage Buffer | 600 μl | 5 ml |
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