Description
TransDirect® Animal Tissue PCR Kit uses a unique lysis buffer to lyse tissues (fresh or frozen). The resulting lysate without purification can be directly used as PCR template. 2×TransDirect® PCR SuperMix (+dye) is highly resistant to various PCR inhibitors present in animal tissues. PCR product can be directly used for gel electrophoresis. AD3 Buffer is recommended to use in aliquots.
Applications
- Direct amplification from unpurified lysate. Suitable for high throughput screening.
- Suitable for mammalian cells, blood, saliva, hair and animal tissues (mammals, marine animals, insects) etc.
- Amplification of genomic DNA fragment up to 3 kb.
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
Product Composition
| Component | AD201-01 | AD201-02 |
| AD1 Buffer | 4 ml | 20 ml |
| AD2 Buffer | 1 ml | 5 ml |
| AD3 Buffer | 4 ml | 2×10 ml |
| 2×TransDirect® PCR SuperMix (+dye) | 1 ml | 5×1 ml |
| Nuclease-free Water | 5 ml | 25 ml |
References
1 Wang S, Teng D, Li X, et al. The evolution and diversification of oakleaf butterflies[J]. Cell, 2022.(IF 66.85)
2 Hou G, Zhou T, Xu N, et al. Integrative Functional Genomics Identifies Systemic Lupus Erythematosus Causal Genetic Variant in the IRF5 Risk Locus[J]. Arthritis & Rheumatology, 2023.(IF 15.48)
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