Human IL-10 ELISA Kit

Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is a pleiotropic cytokine that can exert immunosuppressive or immunostimulatory effects in various types of cells. IL-10 is a homodimeric secretion, mainly produced by monocytes, T cells (mainly Th1 cells), B cells, NK cells, and macrophages. IL-10 has broad anti-inflammatory activity and inhibits the expression of many pro-inflammatory factors, chemokines, and chemokine receptors. IL-10 inhibits the production of IFN-γ and IL-2 by Th1 lymphocytes and the production of IL-4 and IL-5 by Th2 lymphocytes. IL-10 can also inhibit the expression of cell surface antigens, such as CD23, CD80, CD86, etc.

This kit uses Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) to measure the amount of IL-10 in human serum, plasma, and cell culture supernatant. The ELISA microplate in the kit is precoated with a high-affinity anti-human IL-10 antibody. The standard or test sample is added to the microplate wells. After incubation, IL-10 resent in the sample will be bound specifically to the precoated antibody on the microplate wells. After washing, biotin-labeled anti-human IL-10 detection antibody is added to the microplate wells. After re-incubation, the detection antibody will be bound specifically to IL-10 anchored on the microplate wells. Subsequently, the horseradish peroxidase-labeled streptavidin (Streptavidin-HRP) is added to the microplate wells and incubated. Biotin and streptavidin on the detection antibody produces the “coated antibody-human IL-10 protein-detection antibody-Streptavidin-HRP” immune complex through high-strength non-covalent binding. After washing again, the chromogenic substrate TMB is added to the wells. HRP catalyzes the TMB substrate to create blue coloration, with intensity positively correlated with the concentration of IL-10 in the sample. The reaction is stopped by adding stop solution, and the absorbance is measured at 450 nm (reference wavelength 570-630 nm). A standard curve is drawn to calculate the concentration of IL-10 in the sample based on the absorbance value. This kit is highly specific, has high detection sensitivity and is convenient to use.

at 2~8°C in the dark for one year.

ice bag (4℃)