Human FGF basic ELISA Kit

Basic fibroblast growth factor (FGF basic), also known as bFGF, FGF2 or FGF-β, is a member of the fibroblast growth factor family. FGF basic is a wide-ranging growth factor that stimulates the growth of various types of cells such as fibroblasts, osteoblasts, neuronal cells, endothelial cells, keratinocytes, and chondrocytes. bFGF can not only stimulate the formation of new blood vessels, participate in wound healing and tissue reorganization, and promote the development and differentiation of embryonic tissues, but also is closely related to the occurrence and development of tumors.

This kit uses Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) to measure the amount of FGF basic in human serum, plasma, and cell culture supernatant. The ELISA microplate in the kit is precoated with a high-affinity anti-human FGF basic antibody. The standard or test sample is added to the microplate wells. After incubation, FGF basic present in the sample will be bound specifically to the precoated antibody on the microplate wells. After washing, biotin-labeled anti-human FGF basic detection antibody is added to the microplate wells. After re incubation, the detection antibody will be bound specifically to FGF basic anchored on the microplate wells. Subsequently, the horseradish peroxidase-labeled streptavidin (Streptavidin-HRP) is added to the microplate wells and incubated. Biotin and streptavidin on the detection antibody produces the “coated antibody-human FGF basic protein-detection antibody-Streptavidin-HRP” immune complex through high-strength non-covalent binding. After washing again, the chromogenic substrate TMB is added to the wells. HRP catalyzes the TMB substrate to create blue coloration, with intensity positively correlated with the concentration of FGF basic in the sample. The reaction is stopped by adding stop solution, and the absorbance is measured at 450 nm (reference wavelength 570-630 nm). A standard curve is drawn to calculate the concentration of FGF basic in the sample based on the absorbance value. This kit is highly specific, has high detection sensitivity and is convenient to use.

at 2~8°C in the dark for one year.

ice bag (4℃)