Description
This kit is designed for the rapid extraction of total RNA from cultured animal cells, tissues and E. coli. The samples are lysed with guanidine isothiocyanate, DNA is digested with DNase I, and RNA is specifically bound to a silica gel membrane spin column. The extracted total RNA is of high purity, free from DNA and protein contamination, and can be used for RT-PCR, qRT-PCR, microarray analysis, Northern blot, and other experiments.
Storage
DNase I and DNase I Reaction Buffer are at -18℃or below for one year; others are at room temperature (15℃-30℃) in a dry place for one year.
Shipping
Dry ice (-70℃)
Product Composition
| Component | ER101-01(50 rxns) |
| Binding buffer 4 (BB4) | 40 ml |
| Clean buffer 4 (CB4) | 60 ml |
| Wash buffer 4 (WB4) | 12 ml |
| Proteinase K (20 mg/ml) | 1 ml |
| DNase I (3 units/μl) | 1500 U |
| DNase I Reaction Buffer | 4×1 ml |
| RNase-free Water | 10 ml |
| RNase-free Tube (1.5 ml) | 50 each |
| RNA Spin Columns with Collection Tubes | 50 each |
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