Description
This product is universal column-based plasmid maxiprep kit. It uses a modified lysis system to lyse E.coli cells to efficiently release plasmid DNA, followed by the binding of the DNA onto the silica membrane spin column. Lysis and neutralization of 100-500 ml E.coli liquid cultured in LB medium can be completed in a single centrifuge tube. The volume of the supernatant is less than 50 ml. The solution contains indicators that can indicate whether the lysis and neutralization are complete through the change of color, so as to visualize the operation. The new endotoxin removal solution can reduce endotoxin levels to as low as 0.001 EU/μg. The purified DNA is suitable for restriction enzyme digestion, ligation, transformation, sequencing, and transfection, etc.
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