Description
This product is universal plasmid maxiprep kit. It uses a modified lysis system to lyse E.coli cells to efficiently release plasmid DNA, followed by the binding of the DNA onto the silica membrane spin column. Lysis and neutralization of 100-500 ml E.coli liquid cultured in LB medium can be completed in a single centrifuge tube, the volume of the supernatant is less than 50 ml. The solution contains indicators that can indicate whether the lysis and neutralization are complete through the change of color, so as to visualize the operation.The purified DNA is suitable for restriction enzyme digestion, ligation, transformation, sequencing, transfection, etc.
• 1-tube type: Lysis and neutralization of 100-500 ml bacterial liquid can be completed in a single 50 ml centrifuge tube, without the need to prepare large-volume consumables.
• Visualization: Solution LB II (blue) indicates whether lysis and neutralization are complete through the change of color, ensuring the quality of plasmid extraction.
• Fast: The universal system is compatible with extraction of 100-500 ml bacterial liquid. Small volume system combined with large volume spin column greatly reduces the number of centrifugation and saves operation time.
• High yield: Up to 5 mg nucleic acid load in the purification column.
at 15℃-30℃ under dry condition for one year.
Room temperature
| Component | EM152-01 (10 rxns) |
| Resuspension Buffer II (RB II) | 140 ml |
| Lysis Buffer II (LB II, Blue) | 70 ml×2 |
| Neutralization Buffer II (NB II) | 140 ml |
| Activation Buffer II (AB II) | 55 ml |
| Wash Buffer II (WB II) | 25 ml |
| Elution Buffer (EB) | 31 ml |
| RNase A (10 mg/ml) | 1.4 ml |
| Maxi-Plasmid Spin Column with Collection Tube | 10 each |
| Push Filter | 10 each |
| 50 ml Collection Tube | 10 each |
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