DNase I (RNase-free )

Description

Deoxyribonuclease I (DNase I) is an endonuclease that digests single- and double-stranded DNA and chromatin (reaction rate is restricted by DNA association with histones). It functions by hydrolyzing phosphodiester bonds, producing mono and oligodeoxyribonucleotides with a 5′-phosphate and a 3′-hydroxyl group. Its activity depends on Ca²⁺ and it is activated by Mg²⁺ or Mn²⁺. DNase I with Mg²⁺ cleaves randomly double-stranded DNA at any site. DNase I with Mn²⁺ cleaves two DNA strands at approximately the same site to form sticky ends with 1-2 nucleotide overhangs or form blunt ends.

Concentration: 3 units/μl

Storage
at -18℃ or below for two years

Shipping
Dry ice (-70℃).