2×TransStart® GoldPfu PCR SuperMix (-dye)

This product is a ready-to-use mixture of TransStart® GoldPfu DNA polymerase, dNTPs, and optimized reaction buffer showing high amplification efficiency, fast amplification, ultra high fidelity and high specificity. The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and Nuclease-free Water. The amplified products with blunt ends can be directly cloned into pEASY®-Blunt vectors. The SuperMix without dye (-dye) cannot be directly loaded on agarose gel for electrophoresis.

Its PCR product is not suitable for polyacrylamide gel electrophoresis.

• Reduced PCR operation time.
• Avoid contamination due to multi-step operation.
• 54-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.

Highlights

Fast amplification, ultra high fidelity, high specificity, high stability

Applications

• Ultra High fidelity PCR
• Site-directed mutagenesis
• Blunt end cloning
• Complex templates
• GC/ AT rich templates
• Long fragment PCR

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)