TransDetect® Dual-Luc Pro Luciferase Reporter Assay Kit

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Catalog Number:FR203-01

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Description

TransDetect® Dual-Luc Pro Luciferase Reporter Assay Kit provides dual luciferase reporter gene assay system with high-sensitivity, no irritating smell, very stable and homogeneous fluorescence signal. The kit contains high-purity firefly luciferin and coelenterazine, which uses firefly luciferin as a substrate to detect the activity of the firefly luciferase, while quenching the fluorescence reaction, the activity of the Renilla luciferase reporter gene is detected by using coelenterazine as a substrate, thereby achieving detection of two reporter genes in a single sample. Compared to flash-type TransDetect® Double-Luciferase Reporter Assay Kit(FR201), this product is easier to use, enables add-read assay protocol without the need for removal of the medium, and provides more stable glow-type signal.

Features

• Stable luminescent signal: Half-life is about 2 h (20-25℃), suitable for high-throughput detection.

• Accurate result: The Renilla luciferase gene is used as an internal control to correct for variations in cell number between wells and transfection efficiency.

• Easy to use: Without the need for removal of the medium, and the detection reagent can be added directly.

• No irritating smell.

Storage

The unopened kit can be stored at -18℃ or below for one year. The reconstituted Dual-Luc Pro Luciferase Reaction Reagent can be stored at -70℃ or below for one year, or at -18℃ or below for no more than one month, and avoid repeated freezing and thawing. Dual-Luc Pro Luciferase Reaction Reagent II should be freshly prepared before use.

Shipping

Dry ice (-70℃)

Product Composition
Component FR203-01 (100 rxns) FR203-02 (1000 rxns)
Dual-Luc Pro Luciferase Reaction Buffer 10 ml 100 ml
Dual-Luc Pro Luciferase Reaction Substrate (Lyophilized) 1 vial 1 vial
Dual-Luc Pro Luciferase Reaction Buffer II 10 ml 100 ml
Dual-Luc Pro Luciferase Reaction Substrate II (100×) 100 μl 1 ml

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