Description
TransDetect® In Situ Fluorescein TUNEL Cell Apoptosis Detection Kit provides a precise, simple and low-toxicity way to detect and quantify apoptotic cell death at single cell level in cells and tissues. TdT-mediated dUTP Nick-End Labeling (TUNEL) reaction preferentially labels DNA strand breaks generated during apoptosis with fluorescein-labeled dUTP. The fluorescein labeled DNA can be detected and quantified by fluorescence microscopy or flow cytometry. This kit can be used to detect apoptosis in paraffin-embedded tissue sections, cryopreserved tissue sections, cells cultured on chamber slides, cell smear and cell suspensions.
• Extremely low toxicity, labeling solution is free of commonly used highly-toxic cacodylate.
• High sensitivity, high specificity, optimal ratio of labeled and non-labeled substrates.
• Simple, one-step labeling by mixing TdT and labeling solution.
• Flexible, can be used in many assay systems
TdT at -20℃ for one year, 1×Labeling Solution at -20℃ in dark for one year
Dry ice (-70℃)
| Component | FA201-01 (25 rxns) | FA201-02 (50 rxns) |
| TdT | 50 μl | 100 μl |
| 1×Labeling Solution | 1.25 ml | 2×1.25 ml |
1 Teng J F, Qin D L, Mei Q B, et al. Polyphyllin VI, a saponin from Trillium tschonoskii Maxim. induces apoptotic and autophagic cell death via the ROS triggered mTOR signaling pathway in non-small cell lung cancer[J]. Pharmacological research, 2019.(IF 5.57)
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