TransDetect® PCR Mycoplasma Detection Kit

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Catalog Number:FM311-01

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Description

TransDetect® PCR Mycoplasma Detection Kit is designed to detect the presence of mycoplasma contamination by PCR in biological materials such as cultured cells. Highly specific primers have been designed to amplify a fragment of 16S rRNA coding DNA that is conserved across all commonly-known mycoplasma species. Using this kit, cell culture supernatants can be tested directly without DNA extraction. The kit features simple operation, fast, high specificity, and high sensitivity. It can detect a total of 54 mycoplasma species, including M.orale, M.pneumoniae, M.hyorhinis, M.arginini, M.fermentans, M.salivarium, M.pirum, M. hominis, A.laidlawii.

Features

• High specificity: Only detect mycoplasma DNA, not eukaryotic and bacterial DNA.

• Simple and easy to use: Requires no genomic DNA extraction, suitable for high-throughput testing of cell samples.

• Positive and negative control: Ensure reliability and accuracy of the results.

Storage

at -18℃ or below for two years

Shipping

Dry ice (-70℃)

Product Composition
Component FM311-01 (100 rxns)
TransDetect® PCR Mycoplasma SuperMix II (2×) 2×750 μl
Myco Primer Mix II 200 μl
Myco Positive Control Template Ⅲ 100 μl
MycoFree Water 1 ml
References

1 Xu D, Zhao H, Jin M, et al. Modulating TRADD to restore cellular homeostasis and inhibit apoptosis[J]. Nature, 2020.(IF 69.50)

2 Zhao N, Wang F, Kong Z, et al. Pseudorabies Virus Tegument Protein UL13 Suppresses RLR-Mediated Antiviral Innate Immunity through Regulating Receptor Transcription[J]. Viruses, 2022.(IF 5.80)

3 Lei Z, Meng H, Liu L, et al. Mitochondrial base editor induces substantial nuclear off-target mutations[J]. Nature, 2022.(IF 69.50)

4 Bai X, Sun P, Wang X, et al. Structure and dynamics of the EGFR/HER2 heterodimer[J]. Cell Discovery, 2023.(IF 38.07)

5 Huang J, Lin Q, Fei H, et al. Discovery of deaminase functions by structure-based protein clustering[J]. Cell, 2023.(IF 65.00)

6 Wang D, Li X, Jiao D, et al. LCN2 secreted by tissue-infiltrating neutrophils induces the ferroptosis and wasting of adipose and muscle tissues in lung cancer cachexia[J]. Journal of Hematology & Oncology, 2023.(IF 28.50)

7 Nian Z, Zheng X, Dou Y, et al. Rapamycin pretreatment rescues the bone marrow AML cell elimination capacity of CAR-T cells[J]. Clinical Cancer Research, 2021.(IF 12.50)

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