Description
The kit is designed to efficiently and quickly prepare DNA library from 1 ng to 1 μg double-stranded DNA for the Illumina high-throughput sequencing platform. The kit completes the DNA fragmentation, end repair and A-tailing in one step. The product can be directly used for adapter ligation without the purification steps. Due to the use of fragmentation enzymes to fragment the genome, libraries of inserts of different sizes can be obtained by adjusting the fragmentation time, which simplifies the operation process and shortens the library preparation time. And the kit is suitable for library construction of DNA from different sources and inputs.
• Applicable to a wide range of sample types.
• High library conversion rate.
• Whole genome sequencing.
• Target gene sequencing.
• Exon sequencing / other targeted capture sequencing.
• Metagenomic sequencing.
at -18℃ or below for one year
Dry ice (-70℃)
| Component | KP231-01(12 rxns) | KP231-02(96 rxns) |
| 10×Fragmentation Buffer II | 60 μl | 480 μl |
| Fragmentation Enzyme Mix II | 120 μl | 960 μl |
| TransNGS® Adapter for Illumina®(16μM) | 60 μl | 480 μl |
| Adapter Dilution Buffer | 600 μl | 5 ml |
| Adapter-ligation Buffer for Illumina® III | 480 μl | 4×960 μl |
| Adapter-ligation Enzyme V | 60 μl | 480 μl |
| TransNGS® Library Amplification SuperMix(2×) | 300 μl | 4×600 μl |
| TransNGS® Universal Primer Mix for Illumina® | 60 μl | 480 μl |
| Library Elution Buffer | 2 ml | 4×4 ml |
| Nuclease-free Water | 1 ml | 5 ml |
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