Description
The kit is designed for the isolation of RNA from various fresh and dried plant samples, including plant samples rich in polysaccharides and polyphenols. The kit is based on unique extraction system and membrane technology to specifically remove secondary metabolites such as polysaccharides, polyphenols, and lipids in samples, without the need for toxic reagents such as phenol and chloroform. Purified RNA with high quality and good stability can be used for RT-PCR, qRT-PCR, Northern blot, RACE, library construction, etc.
• Wide range of applications: applicable to various plant tissues, especially those rich in polysaccharides, polyphenols or starch
• Fast operation: high-quality genomic RNA can be extracted in less than 30 minutes
• Safe and low toxicity: no toxic organic reagents such as phenol, chloroform, or β-mercaptoethanol
• High purity: the unique technology can efficiently remove impurities such as pigments, polyphenols and polysaccharides in the sample
at 15℃-30℃ in a dry place for two years; DNase I at -18°C or below for two years.
DNase I should be transported with dry ice; ther components at room temperature.
| Component | ER302-01 (50 rxns) | ER302-02 (200 rxns) |
| Lysis Buffer 52 (LB52) | 40 ml | 160 ml |
| Precipitation Buffer 52 (PB52) | 10 ml | 40 ml |
| DNase I (3 units/μl) | 1500 U | 6000 U |
| DNase I Reaction Buffer | 4×1 ml | 15 ml |
| Clean Buffer 52 (CB52 ) | 20 ml | 80 ml |
| Wash Buffer 52 (WB52) | 12 ml | 2×22 ml |
| RNase-free Water | 10 ml | 20 ml |
| Filtration Columns with Collection Tubes | 50 | 200 |
| RNA Spin Columns with Collection Tubes Ⅱ | 50 | 200 |
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