Description
EasyPure®Genomic DNA Kit provides a simple and convenient way to efficiently isolate high-quality genomic DNA from a variety of materials (mammalian cells, tissues, mouse tails, E. coli and yeast). Cells and tissues are enzymatically lysed. DNA is specifically bound to silica-based column. The isolated DNA is suitable for PCR, restriction enzyme digestion and Southern blot, etc.
• Fast extraction, high yield (up to 15 μg).
• High integrity of cDNA ensured by mild lysis conditions without physical disruption, thus reducing damage to gDNA during cell lysis process
• High purity enabled by spin column which can efficiently and specifically bind to DNA and removs protein, salts, lipids or other contaminants.
At room temperature (15-25℃) in a dry place for one year.
At room temperature.
Research Use Only
| Component | EE101-01/-11 (50 rxns) | EE101-02/-12 (200 rxns) |
| Lysis Buffer 2 (LB2) | 6 ml | 24 ml |
| Binding Buffer 2 (BB2) | 28 ml | 110 ml |
| Clean Buffer 2 (CB2) | 15 ml | 60 ml |
| Wash Buffer 2 (WB2) | 12 ml | 2×22 ml |
| Elution Buffer (EB) | 25 ml | 80 ml |
| RNase A (20 mg/ml) | 1 ml (EE101-01)/0 (EE101-11) | 4×1 ml (EE101-02)/0 (EE101-12) |
| Proteinase K (20 mg/ml) | 1 ml | 4×1 ml |
| Genomic Spin Columns with Collection Tubes | 50 each | 200 each |
1 Liu S, Zhang M, Bao Y, et al. Characterization of a Highly Selective 2 ″-O-Galactosyltransferase from Trollius chinensis and structure-guided engineering for improving UDP-glucose selectivity[J]. Organic Letters, 2021.(IF 6.00)
2 Li W, Ali T, Zheng C, et al. Anti-depressive-like behaviors of APN KO mice involve Trkb/BDNF signaling related neuroinflammatory changes[J]. Molecular Psychiatry, 2022.(IF 15.99)
3 Wei G, Sun H, Dong K, et al. The thermogenic activity of adjacent adipocytes fuels the progression of ccRCC and compromises anti-tumor therapeutic efficacy[J]. Cell metabolism, 2021.(IF 27.28)
4 Song X, Yang F, Yang T, et al. Phase separation of EB1 guides microtubule plus-end dynamics[J]. Nature Cell Biology,2023.(IF 28.21)
5 She X, Shen S, Chen G, et al. Immune surveillance of brain metastatic cancer cells is mediated by IFITM1[J]. The EMBO Journal, 2023.(IF 14.01)
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