Description
TransStart® FastPfu PCR SuperMix is a ready-to-use mixture of TransStart® FastPfu DNA polymerase, dNTPs, and optimized buffer. The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and H2O.
• TransStart® FastPfu PCR SuperMix offers 54-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 2-4 kb/min.
• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.
• High fidelity PCR
• High yield PCR
• Fast PCR
• Blunt end cloning
• Site-directed mutagenesis
• Complex templates
at -20 ℃ for two years
Dry ice (-70 ℃)
| Component | AS221-01 | AS221-02 |
| 2×TransStart® FastPfu PCR SuperMix (-dye)/ (+dye) | 1 ml | 5×1 ml |
| Nuclease-free Water | 1 ml | 5 ml |
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3 Chen K, Hu Z, Song W, et al. Diversity of O-glycosyltransferases contributes to the biosynthesis of flavonoid and triterpenoid glycosides in Glycyrrhiza uralensis[J]. ACS Synthetic Biology, 2019.(IF 5.57)
4 Wang Y, Wang Z, Chen Y, et al. A highly efficient CRISPR-Cas9-based genome engineering platform in Acinetobacter baumannii to understand the H2O2-sensing mechanism of OxyR[J]. Cell Chemical Biology, 2019.(IF 6.76)
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