2×TransTaq® High Fidelity (HiFi) PCR SuperMix I (-dye)

TransTaq® High Fidelity (HiFi) PCR SuperMix I or II is a ready-to-use mixture of TransTaq® High Fidelity (HiFi) DNA polymerase, dNTPs and optimized buffer. TransTaq® High Fidelity (HiFi) PCR SuperMix I is optimized for the amplification of genomic DNA and PCR SuperMix II is optimized for the amplification of λDNA, cDNA or plasmid DNA. The SuperMix is  provided at 2× concentration and can be used at 1× concentration by adding template, primers and H2O.

• TransTaq® High Fidelity (HiFi) PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.

• Extension rate is about 1-2 kb/min.

• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.

• Amplification of genomic DNA fragment up to 15 kb.

Applications

• Complex templates

• GC/AT-rich templates

• Long PCR

• High yield PCR

Storage

at -20 ℃ for two years

Shipping

Dry ice (-70 ℃)