Description
TransStart® TopTaq DNA Polymerase is an engineered version of Taq DNA Polymerase combined with TransStart® technique. One binding protein binds to double-strand DNA template, preventing polymerase activity at room temperature. Other two binding proteins bind primers, preventing primer-dimer formation. Blocking proteins are released from primers and templates during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.
- Compared with TransStart® Taq DNA Polymerase, TransStart®TopTaq DNA Polymerase has higher amplification efficiency, specificity and sensitivity.
- TransStart® TopTaq DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
- The specificity is higher than antibody based or chemically modified hot start DNA polymerases.
- Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
- Reduced nonspecific amplification and primer dimer formation.
- Different from Taq antibody, no risk of contamination from mammalian DNA.
- Different from chemical modification, long denaturing step is not needed.
- Amplification of genomic DNA fragment up to 15 kb.
Applications
- Complex templates
- GC/AT-rich templates
- Multiplex PCR
- High yield PCR
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
Product Composition
| Component | AP151-01/11 | AP151-02/12 | AP151-03/13 |
| TransStart® TopTaq DNA Polymerase | 250 U×1 | 500 U×1 | 500 U×6 |
| 10×TransStart® TopTaq Buffer | 1.2 ml | 1.2 ml×2 | 1.2 ml×12 |
| 2.5 mM dNTPs | -/800 μl×1 | -/800 μl×2 | -/1.2 ml×8 |
| 10×GC Enhancer | 200 μl×1 | 400 μl×1 | 1 ml×1 |
| 6×DNA Loading Buffer | 500 μl×1 | 1 ml×1 | 1 ml×2 |
References
Su Y , Li B , Zhu W Y . Fecal microbiota of piglets prefer utilizing dl-lactate mixture as compared to d-lactate and l-lactate in vitro[J]. Anaerobe, 2013, 19(Complete):27-33.
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