Description
TransDirect® Mouse Genotyping Kit uses a unique lysis buffer to prepare mouse genotyping PCR-ready DNA from fresh or frozen mouse tissue slices, such as mouse ears, toes and tails. The high-efficient 2×TransDirect® Mouse Genotyping SuperMix (+dye) can effectively suppress the inhibitory activities of the crude lysate for PCR amplification.
Applications
- Direct PCR amplification from crude lysate.
- Suitable for PCR-based rapid mouse genotyping.
- Easy-to-use and suitable for high-throughput applications.
- Suitable for multiplex PCR with up to 5 pairs of primers.
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
Product Composition
| Component | AD501-01 (100 rxns) | AD501-02 (500 rxns) |
| AD1 Buffer | 12 ml | 60 ml |
| AD2 Buffer | 3 ml | 15 ml |
| 2×TransDirect® Mouse Genotyping SuperMix (+dye) | 1 ml | 5×1 ml |
| 10×GC Enhancer | 1 ml | 5×1 ml |
| Nuclease-free Water | 5 ml | 25 ml |
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