Description
TransDirect® Plant Tissue PCR Kit uses a unique lysis buffer to lyse plant tissues (fresh or frozen). The resulting lysate without purification can be directly used as PCR template. 2×TransDirect® PCR SuperMix (+dye) is highly resistant to various PCR inhibitors present in plant tissues. PCR product can be directly used for gel electrophoresis.
- No DNA extraction needed.
- Ready-to-use SuperMix.
- DNA fragment up to 2 kb.
- For plants with moderate contents of polysaccharides or polyphenols.
Applications
- Direct amplification from unpurified lysate. Suitable for high throughput applications.
- Amplification of genomic DNA fragment up to 2 kb.
Storage
2×TransDirect® PCR SuperMix (+dye) at -20 ℃ for two years, others at room temperature (15-25 ℃) for two years.
Shipping
2×TransDirect® PCR SuperMix (+dye) on dry ice (-70 ℃); others at room temperature.
Product Composition
| Component | AD301-01 | AD301-02 |
| PD1 Buffer | 4 ml | 20 ml |
| PD2 Buffer | 4 ml | 20 ml |
| 2×TransDirect® PCR SuperMix (+dye) | 1 ml | 5×1 ml |
| Nuclease-free Water | 5 ml | 25 ml |
References
1 Liu H, Li J, Gong P, et al. The origin and evolution of carpels and fruits from an evo‐devo perspective[J]. Journal of Integrative Plant Biology, 2023.(IF 6.99)
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