Description
EasyTaq® DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. It lacks 3′-5′ exonuclease activity. EasyTaq® DNA Polymerase is suitable for routine amplification. PCR products are unsuitable for PAGE.
• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 4 kb.
Applications
• Routine PCR
• High throughput PCR
• Colony PCR
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
Without dNTPs
| Component | AP111-01 | AP111-02 | AP111-03 | AP111-04 |
| EasyTaq® DNA Polymerase | 500 U×1 | 500 U×6 | 2500 U×4 | 5000 U×10 |
| 10×EasyTaq® Buffer | 1.2 ml×1 | 1.2 ml×6 | 1.2 ml×20 | 1.2 ml×100 |
| 6×DNA Loading Buffer | 1 ml×1 | 1 ml×2 | 1 ml×4 | 1 ml×20 |
With dNTPs
| Component | AP111-11 | AP111-12 | AP111-13 |
| EasyTaq® DNA Polymerase | 500 U×1 | 500 U×6 | 2500 U×4 |
| 10×EasyTaq® Buffer | 1.2 ml×1 | 1.2 ml×6 | 1.2 ml×20 |
| 2.5 mM dNTPs | 800 μl×1 | 800 μl ×6 | 800 μl×20 |
| 6×DNA Loading Buffer | 1 ml×1 | 1 ml×2 | 1 ml×4 |
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