Description
TransFast® Taq DNA Polymerase is an engineered version of Taq DNA Polymerase. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. TransFast® Taq DNA Polymerase has 5’-3’ DNA polymerase activity and 5’-3’ exonuclease activity. It lacks 3’-5’exonuclease activity.
• Extension rate is about 6 kb/min.
• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of Tgenomic DNA fragment up to 4 kb.
• Routine PCR
• High throughput PCR
• Colony PCR
at -20 ℃ for two years
Dry ice (-70 ℃)
| Component | AP101-01/11 | AP101-02/12 |
| TransFast ® Taq DNA Polymerase | 500 U×1 | 500 U×6 |
| 10×TransFast ® Taq Buffer | 1.2 ml ×1 | 1.2 ml ×6 |
| 2.5 mM dNTPs | – / 800 μl ×1 | – / 800 μl ×6 |
| 6×DNA Loading Buffer | 1 ml×1 | 1 ml ×2 |
References
1 Chen J, Hu X, Shi T, et al. Metabolite‐based genome‐wide association study enables dissection of the flavonoid decoration pathway of wheat kernels[J]. Plant Biotechnology Journal, 2020.(IF 6.84)
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